Cccp protonophore
WebAug 14, 2001 · We have previously shown that treatment of human osteosarcoma cells with the protonophore m -chlorophenylhydrazone (CCCP) for 6 h induces MPT and mitochondrial swelling without significant cell death. Here we demonstrate that release of cytochrome c does not occur and the cells remain viable even after 72 h of treatment … WebWe propose a simple model that accounts for the ability of the weak acid FCCP (Carbonylcyanide-p-trifluoromethoxyphenylhydrazone) to both transport protons across ...
Cccp protonophore
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WebNov 1, 2013 · Depolarization of mitochondria by the protonophore CCCP is a strategy increasingly used to experimentally trigger not only mitophagy, but also bulk autophagy. … WebWe manufacture high quality biochemicals, assay kits, antibodies, and recombinant proteins and offer contract services for custom chemical synthesis/analysis, assay …
WebAs expected of a protonophore, CCCP (a) induced swelling in mitochondria suspended in potassium ace- tate and valinomycin, (e) induced rapid K+ efflux from mitochondria treated with valinomycin, and (c) caused an €!-fold stimulation of respiration. Butacaine alone was unable to mimic these effects of CCCP; however, WebTreatment of acrA strain CL2 with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) dissipated delta psi, decreased intracellular ATP levels, and resulted in cessation of growth; after a variable period of time (3 to 7 h), growth resumed, ultimately achieving growth rates comparable to those of untreated cells.
WebCarbonyl cyanide 3-chlorophenylhydrazone (CCCP) induces mitophagy in mammalian cells. CCCP suppresses the expression of few electron transport chain (ETC) proteins. Protonophore (H + ionophore) and uncoupler of oxidative phosphorylation in mitochondria. Shown to have a number of effects on cellular calcium. WebWe used the protonophore CCCP to depolarize mitochondria and to trigger mitochondrial translocation of Parkin. Cells are then fixed and high-throughput immunofluorescence …
WebCCCP is a protonophore. It disrupts mitochondria membrane potential by transporting protons across the mitochondrial inner membrane, which interferes with the proton gradient and ATP synthesis. CCCP is a widely used as an uncoupler of oxidative phosphorylation to induce mitochondrial depolarization.
WebApr 23, 2013 · The stimulating action of penetrating cations on the protonophore-mediated uncoupling is assumed to be useful for medical applications of low (non-toxic) concentrations of protonophores. ... Note that the steady-state level of electric current with C 12 R4 was negligible if no protonophore (CCCP) was added. The current was higher in … the copeland gangWebThe product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria the cope meaningWebProtonophore. Uncoupling agent for oxidative phosphorylation that inhibits mitochondrial function. Approximately 100 times more effective than 2,4-dinitrophenol. Synonym (s): … the cope of night bgmWebThese data support an autosomal dominant mitochondrial uncoupling syndrome with hypermetabolism. (Funded by the National Institutes of Health.) Figure 1. Oxidative Phosphorylation in Normal and ... the copenhagen cloudburst formulaWebFeb 14, 2024 · Protonophores (e.g., CCCP) reduce ATP production and increase membrane permeability in bacteria ( Spindler et al., 2011; Park and Ko, 2015; Mohamed et al., 2016; … the copenhagen companyWebCarbonyl cyanide 3-chlorophenylhydrazone (CCCP) induces mitophagy in mammalian cells. CCCP suppresses the expression of few electron transport chain (ETC) proteins. … the copenhagen general population studyWebDepolarization of mitochondria by the protonophore CCCP is a strategy increasingly used to experimentally trigger not only mitophagy, but also bulk autophagy. the cope twins