Cls frtl-5
WebOct 19, 2024 · Using the Fischer rat thyroid follicular (FRTL-5) cell line which endogenously expresses NIS, we evaluated the FRTL-5 in the RAIU as a secondary screening tool to further clarify and prioritize potential NIS inhibitors. Following assay validation, results from controls, reference and chemical tests indicated a highly robust and reproducible ... WebJun 21, 2024 · The FRTL-5 RAIU assay was optimized in a 96-well format over a 2-hr exposure period, and validated with a set of known NIS inhibitors and inactive compounds. The 12 top-ranked chemicals (ranking scores 58.5 - 150.4, relative to a score of 200 for sodium perchlorate) of the HTPS hNIS RAIU assay were then tested in the FRTL-5 RAIU …
Cls frtl-5
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WebFeb 1, 2007 · Growth rates of FRTL-5 cells stably transfected with Wnt-1 expression vector (W1) or control vector (V3) were compared in the presence of staurosporin or control (DMSO) using MTT assay. Background ... WebMay 24, 2024 · Hello, I Really need some help. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. I pretty …
WebFigure Legend Snippet: Secretion of thyroglobulin and expression of ZnT transporters in FRTL-5 cells. ( A ) Confluent cells were grown for 24 h in the presence (+) or absence (-) of serum (FBS) and/or a hormone cocktail. Supernatants were collected after 2, 4, 8, and 24 h and analyzed by Western blotting with anti-thyroglobulin (Tg) antibodies. Web144 Exp. Mol. Med. Vol. 31(3), 142-150, 1999 for at least 1 h. Precipitated DNA was collected by centrifugation at 14,000 g for 30 min at 4oC, washed with ice-cold 80% ethanol and air-dried.
WebFRTL-5 rat thyroid epithelial cells maintain normal thyroid function and morphology in vitro, exhibit an absolute requirement for thyroid stimulating hormone (TSH) for proliferation and display radiation dose response characteristics indistinguishable from those of rat thyroid epithelial cells in vivo. In TSH-free medium cells remain in a non ... WebCLS Cell Lines Service GmbH. view supplier details. Catalog No: 500407. Size cryovial cryovial; Please select Size. Quantity. Price. $910 ... Rat Thyroidea cell line FRTL-5 Growth Properties Adherent, clumps with raised centers ...
WebBarae Jomaa, in Toxicogenomics-Based Cellular Models, 2014. In vitro models for the inhibition of iodide transport. The epithelial follicular rat thyroid cell line FRTL-5 is the …
WebApr 1, 1992 · TSH-mediated reduction of MHC class I gene transcription in FRTL-5 cells was mapped to a region within 135 base pairs of the promoter. TSH-mediated decreases in class I RNA levels are duplicated ... helm snailWebFRTL-5 cells, a diploid line of differentiated rat thyroid epithelial cells, have been grown as multicellular spheroids in spinner culture. Spheroids were initiated by seeding FRTL-5 cells either into Lab-Tek dishes or culture flasks with a 0.5% agar base. Thyroid stimulating hormone (TSH, >1.0 mU/ml) was required for initial cell aggregation and spheroid … helmsman job in singaporeWebFRTL-5 Zelllinien online kaufen Zellkulturen CLS.shop Sie sind hier: Produkte Zelllinien Tierische Zelllinien Ratte Zelllinien FRTL-5 slide 2 of 6 540,00 €* Inhalt: 1 Kryovial Preise … helmsman ptaWebApr 22, 2024 · While treatment of FRTL-5 cells with TM alone (0.1 µg/mL) for 48 h strongly induced the ER stress-sensitive genes heat shock protein family A member 5 (HSPA5) and DNA damage inducible transcript ... helmsman mayonnaiseWebFRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features … helmsley hotel sarasotaWebFRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. FRTL-5 cells have been used as assays measuring the stimulatory activity of human autoimmune ... helmsman stain sealerWebApr 29, 2024 · FRTL-5 cells were transfected with the pSV0-CAT vector, as described above for the PGL3 vectors, and treated with quercetin 10 μM for 48 h. As shown in Figure 2, quercetin treatment increased 10-fold the CAT expression compared to control activity, 1135.5% ± 24.5% of control. Surprisingly, this effect was even higher than that observed … helmsman matte