Dh5 alpha subcloning efficiency
WebDec 13, 2011 · E. coli. (Subcloning Efficiency)? The properties of this strain that contribute to its usefulness as a cloning strain are described below. The genotypes underlying … WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : …
Dh5 alpha subcloning efficiency
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WebDescription. Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10 6 cfu/μg … WebSep 8, 2015 · To determine the efficiency and fidelity of DH5α-mediated in vivo assembly and cloning, we designed a pUC19 plasmid-based screen . In this work, efficiency is …
WebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. Transformation efficiency should be greater than 1 x 106 transformants/µg pUC19 DNA. … WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5-alpha Competent E. coli (Subcloning Efficiency) C2988: 6 x 0.4ml: MAX Efficiency ® DH5αF'IQ ™ Competent Cells* ThermoFisher (Invitrogen) 18288-019: 5 x 0.2ml
WebDH5-alpha Comp Cells SUBCLONING 500 ul # 18265-017: each: Life Technologies: 57648: DH5-alphaCompCells MAX Efficiency 200ul #18258012: each: Life Technologies: 57649: BL21 STAR (DE3) OneShot CompCells 50 ul #C6010-03: ... NEB 5-alpha Competent E.Coli 20 x .05 ml # C2987H: box: WebE. coli Competent Cells are prepared according to a modified procedure of Hanahan. The competent cells can be used for many standard molecular biology applications. JM109 competent cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 10 8 cfu/µg and Subcloning Efficiency at greater than 10 7 ...
Webtransformation efficiency. The stock solution of pFastBac™-gus (0.2 g/ml), µ provided with pFastBac™1 Expression Vector (Cat. No. 10360-014), can be used as a control for the transposition frequency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. 1.
WebAug 2, 2024 · This product has been discontinued. Alternatives are NEB® 10-beta Electrocompetent E. coli ( NEB #C3020K) or chemically competent cells, NEB® 5-alpha Competent E. coli (High Efficiency) ( NEB #C2987) Catalog # C2989 was discontinued on August 02, 2024. NEB 5-alpha Electrocompetent Competent E. coli is a derivative of the … citicards luke bryan presaleWebChemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency of these cells is not sufficiently high for most other cloning applications. For that, we recommend NEB® 5-alpha Competent E. coli (High … diaphoresis what is itWebThermo Scientific™ DH5α Competent Cells. Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. Brand: Thermo Scientific™ EC0111. Additional Details : Weight : 17.00000kg. Product Code. 16506900. £50.25 / 40 reactions. citicards login to my accountWebDH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications: DNA cloning following PCR or restriction digestion reactions. Subcloning (transferring DNA from a parent vector to a destination vector) Plasmid isolation. Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 … diaphoresis vs sweatsWebDec 13, 2011 · E. coli. (Subcloning Efficiency)? The properties of this strain that contribute to its usefulness as a cloning strain are described below. The genotypes underlying these properties appear in parentheses. Blue/White Screening: (Φ80 Δ (lacZ)M15 makes the omega-fragment of β-galactosidase (β-gal); (argF-lacZ) deletes the β-gal gene on the ... diaphoresis wikipediaWeb5 Using DH5α-T1R as a Transient Host Introduction DH5α-T1R competent E. coli support replication of M13mp vectors, but it does not support plaque formation. Plating on a lawn of E. coli containing the F plasmid will allow plaque formation. Before Starting You will need the following reagents: • Log-phase E. coli containing the F plasmid (e.g. DH5α-FT™, … citi cards mailing addressWebSubcloning Efficiency™ DH5α™ Chemically Competent E. coli Catalog no. 18265-017 General Information and Kit Contents Shipping and Storage The kit is shipped on dry ice. … citicards mailing address for payment