Hoxb8-fl
WebTo test if Cas9-Hoxb8 cell-derived DCs rely on the same mechanisms and thus can be exploited as a tool to study DC migration, we used lentiviral-based CRISPR/Cas9 … BM– and Hoxb8-FL–derived DCs and macrophages were generated by cultivating unfractionated BM cells or Hoxb8-FL cells for 6 d in standard growth medium (RPMI 1640 (Invitrogen) for DCs or DMEM (Invitrogen) for macrophages, supplemented with 10% (v/v) FCS (Hyclone), 50 mM 2-mercaptoethanol and … Meer weergeven Antibodies used were against B220 (RA3-6B2, dilution 1:200), CD4 (RM4-5, dilution 1:200), CD45.1 (A20, dilution 1:200), MHC class II … Meer weergeven BM cells were collected by flushing femurs of 4–8-week-old female C57BL/6J or B6.SJL mice with 10 ml RP-10 (RPMI 1640 … Meer weergeven The plasmids MSCV-ERHBD-Hoxb8 and the ecotropic packaging vector pCL-Eco (Imgenex) were both transfected into HEK293T … Meer weergeven Hoxb8-FL cells and BM cells were differentiated in the presence of M-CSF contained in L-cell conditioned medium for 7 d, followed by incubation with FITC-labeled IgG-coated beads (1:100, Cayman Chemical, … Meer weergeven
Hoxb8-fl
Did you know?
Web29 nov. 2024 · Serial monitoring of peripheral blood demonstrated that Hoxb8-FL derived cells had disappeared by 4 weeks, and did not reappear. However, at 6 months CEBPA N321D-transduced cells could still be detected in bone marrow in contrast to EV-transduced cells but without any leukemic phenotype. WebHoxB8-FL cells differentiate into myeloid, lymphoid and dendritic cells in vitro upon withdrawal of E2, or in vivo upon transplantation. They therefore represent lymphoid-primed multipotent progenitor (LMPP)-like cells that are unable to self-renew in the absence of E2.
WebData are representative for at least five independent populations of Hoxb8–FL cells. ( b ) Three–dimensional PCA based on gene expression profiles of Hoxb8–FL cells (four … Web12 mrt. 2024 · Hoxb8-FL cells can be infected with different retroviruses, including ecotropic MSCV and VSV-G-pseudotyped lentivirus, generated by standard procedures (see Note …
Web26 nov. 2024 · The HoxB8 MPP differentiation system developed here extends previous work on HoxB8 cells and DC differentiation [6, 9 – 11] and generates the full complement of DC subsets, cDC1, cDC2, and pDC. In addition, we established a versatile workflow for gene targeting with CRISPR/Cas9 to study DC differentiation. Web1 jan. 2024 · Flow cytometry and single-cell transcriptomic characterisation of cells at different time points revealed that the mutation stalls the multipotent HOXB8-FL cell line along the differentiation trajectory resulting in a self-renewing progenitor-like state exhibiting haematopoietic markers indicative of a plasmacytoid dendritic cell-like phenotype.
WebHoxb8 is a homeobox gene that inhibits myeloid differentiation. Integration of a murine stem cell virus encoding HoxB8 under the control of mutant estrogen receptor promotes …
WebThrough specific growth factors and a co-culture with stromal cells, HoxB8-FL HPCs can differentiate into various hematopoietic lineages, such as B-and T-lymphocytes [3], dendritic cells [4],... ostra halls and hotelsWeb5 mrt. 2024 · Solene Accarias, Thibaut Sanchez, Arnaud Labrousse, Myriam Ben-Neji, Aurélien Boyance, Renaud Poincloux, Isabelle Maridonneau-Parini, Véronique Le Cabec, Ana-Maria Lennon-Duménil; Genetic engineering of Hoxb8-immortalized hematopoietic progenitors – a potent tool to study macrophage tissue migration. rock band guitar pcWeb1 okt. 2010 · The Hoxb8 - Cre mouse line should be a valuable new tool for the in vivo analysis of peripheral and spinal gene functions in pain pathways. Keywords: spinal cord, … ostrander construction beach lake paWebImportantly, Hoxb8-FL cells can be efficiently targeted via CRISPR mediated gene editing (Leithner et al, Eur J Immunol). Thus, we employ immortalised hematopoietic … rock band hamilton ontarioWeb26 okt. 2024 · Hoxb8-FL cell line is a model system of lympho-myeloid progenitors, which self-renews in vitro and is amenable to genetic perturbations. To construct a functionally … ostrander lake fishingWeb13 feb. 2024 · Hoxb8-FL cells, homozygous for membrane GFP (mG), were infected with lentiviruses coding for puromycin resistance, Cas9 and a guide RNA (gRNA) 13 directed … rock band guitar wirelessWeb3 mei 2024 · Integrin β2 can be substituted by its human homolog in mouse Hoxb8 FL cells. (A) Scheme of workflow. Hoxb8 FL cells were generated from bone marrow of integrin β2-deficient mice and retrovirally transduced with mouse or human integrin β2. Human/mouse integrin β2-positive cells were FACS-sorted. ostrander fillmore county minnesota